Ronald E. Majors

Ron Majors, editor of "Column Watch" and "Sample Prep Perspectives," has been with LCGC North America for over 26 years. Currently a senior scientist with Agilent Technologies, Wilmington, Delaware, Ron is known industry-wide as one of the premier chromatography experts in the field. He is also a member of LCGC's editorial advisory board.

An interview with the inventors about the successes, challenges and potential future directions of this technique.

In this installment, the subject of LPME is reviewed, with emphasis on the use of hollow fibers.

The inventors of the QuEChERS technique present an update and a look at the future.

We focus on the characterization of reversed-phase columns with particular emphasis on the hydrophobic-subtraction model

The coauthors discuss the various attributes of and approaches to reversed-phase chromatography.

We focus on the characterization of reversed-phase columns with particular emphasis on the hydrophobic-subtraction model

The authors review the requirements for columns specifically designed and manufactured for SFC.

Ron Majors reports on some of the improvements that have been made over the years focusing on efficiency, stability, and selectivity.

Improvements in HPLC column technology continue. Since the last Special Supplement on columns in 2008 (1), the continued need for high-throughput separation, the encountering of more complex samples, the need to analyze very polar compounds more easily, and the increased availability of very high pressure instruments has placed additional demands on the HPLC column. In terms of high throughput, no less than 24 companies have responded and have developed small particle columns of 2 µm and under. In addition, the number of superficially porous particle (SPP) columns has expanded, and users are finding that the high-efficiency-at-low-pressure advantage brings them added advantages. With more complex samples, longer columns packed with sub-2-µm particles require higher pressures with pumps now available to 1300 bar. It will be of interest to see if the lower pressure, high-efficiency SPP columns and monoliths, the latter of which have seen advances in the research of polymeric monoliths for applications to..