http://chromatographyonline.findanalytichem.com/lcgc ChromatographyOnline, the website of LC/GC North America, provides applications-based technical information and practical troubleshooting advice to chromatographers and analytical chemists. 2009-07-03T20:59:15Z http://chromatographyonline.findanalytichem.com/lcgc/See-Agilent-SampliQ-QuEChERS-Kits-in-Action/ArticleStandard/Article/detail/606957?ref=25 QuEChERS is the fast, proven method to prepare food for pesticide analysis. Now it's easier than ever to start using it in your lab! 2009-07-03T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/272009/606957null HTML http://chromatographyonline.findanalytichem.com/lcgc/Anatomy-of-an-Ions-Fragmentation-After-Electron-Io/ArticleStandard/Article/detail/607083?ref=25 Determining an organic compound's structure from its mass spectrum is challenging. If, as in the case of soft ionization — chemical ionization (CI), atmospheric pressure chemical ionization (APCI), field ionization (FI), electrospray (ES), atmospheric pressure photoionization (APPI), or fast atom bombardment (FAB) — the mass spectrum only provides the mass of the intact molecule, the challenge can be even greater. At best, an elemental composition can be determined if the measured mass is sufficiently accurate; however, many of these elemental compositions represent large numbers of compounds. There are 127 compounds in the NIST08 Mass Spectral Database that have the elemental composition C10H20O. Without an accurate mass measurement, the challenge is even more overwhelming. The elemental composition C10H20O has a nominal mass of 156 Da. The NIST08 Mass Spectral Database has 684 compounds with this nominal mass. To get past this point of an elemental composition, fragmentation of the ion.. 2009-07-01T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/272009/607083null XML http://chromatographyonline.findanalytichem.com/lcgc/HPLC-2009-Wednesday-Afternoon/ArticleStandard/Article/detail/605612?ref=25 The last of our HPLC 2009 show dailies 2009-07-01T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605612null HTML http://chromatographyonline.findanalytichem.com/lcgc/Improving-the-Analytical-Workflow-for-Protein-Biop/ArticleStandard/Article/detail/607086?ref=25 The ability to characterize protein therapeutics throughout the product development cycle is an important requirement for analytical support in the biopharmaceutical industry. Liquid chromatography–mass spectrometry (LC–MS) has played an important role in the ensemble of analytical tools to generate in-depth characterization data for biotherapeutic drugs. All too often in biopharmaceutical characterization, the quest for high-performance MS, when combined with a goal of routine analytical methods, results in compromises in system performance. This article will present an efficient analytical workflow for protein characterization using LC–MS. The workflow enables scientists to obtain high-performance characterization in a routine manner without compromises. Results on intact protein analysis and peptide mapping for a humanized monoclonal antibody will be presented. The value of using a logical workflow that combines optimized LC instrumentation, columns, MS, and software for targeted data.. 2009-07-01T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/272009/607086null XML http://chromatographyonline.findanalytichem.com/lcgc/Quantitative-Proteomic-Workflow-for-Discovery-of-E/ArticleStandard/Article/detail/607087?ref=25 The accurate diagnosis of renal allograft rejection currently depends upon a biopsy. Transplant medicine would benefit greatly from the availability of noninvasive tests for early detection of rejection and immunosuppressive drug therapeutic monitoring. Only a limited number of studies have been published to date on specific proteins associated with allograft rejection. Typically, renal dysfunction due to humoral transplant rejection or other pathologies results in the increase of protein excreted in urine (1–5). In blood, endogenous peptides (not generated by trypsin digestion ex vivo) are likely candidate biomarkers for many diseases and pathologies as they are secreted from tissues and enter the bloodstream (6,7). The analysis of endogenous protein and peptide fragments in urine can provide a noninvasive, early indication of kidney transplant rejection or disease. 2009-07-01T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/272009/607087null XML http://chromatographyonline.findanalytichem.com/lcgc/Application-of-Microbore-UHPLCndashMS-MS-to-the-Qu/ArticleStandard/Article/detail/607085?ref=25 Liquid chromatography coupled with tandem mass spectrometry (LC–MS-MS) is the primary bioanalytical technique used today within the pharmaceutical industry for the quantitation of small molecules in biological matrices such as plasma. In recent years, chromatographic resolution has been improved with the development of ultrahigh-pressure liquid chromatography (UHPLC) systems that utilize smaller diameter particles (< 3 μm) and operate at pressures > 5000 psi. While most LC systems utilized for this application are optimized for columns with internal diameters (i.d.) between 2.0 and 4.6 mm, a new UHPLC system designed for microbore columns (≤ 1 mm i.d.) has been introduced recently. This article will discuss the advantages of using a microbore UHPLC system coupled with a tandem mass spectrometer for the quantitation of in vivo pharmacokinetic samples. These advantages include reduced sample and solvent consumption, improved chromatographic resolution and speed, and reduced mass spectrometer source.. 2009-07-01T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/272009/607085null XML http://chromatographyonline.findanalytichem.com/lcgc/ADMEPharmacokinetic-Studies-from-Serum-and-Plasma-/ArticleStandard/Article/detail/607084?ref=25 The paramount problem in performing absorption, distribution, metabolism, and excretion (ADME)/pharmacokinetic studies of therapeutic oligonucleotides revolves around inefficient and labor-intensive sample preparation. These traditional methods require multiple steps — liquid–liquid extraction (LLE) and solid-phase extraction (SPE) — to extract therapeutic oligonucleotides from serum and plasma for liquid chromatography–mass spectrometry (LC–MS) analysis and thus are not practical for clinical studies with large numbers of samples. Furthermore, these methods tend to yield low recoveries and poor reproducibility. This article presents a revolutionary new method for performing sample cleanup of therapeutic oligonucleotides from serum and plasma. The method extracts many types of therapeutic oligonucleotides from biological matrices in a rapid four-step SPE protocol that eliminates the need for LLE and can be automated for large sample sets. In the testing presented, different.. 2009-07-01T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/272009/607084null XML http://chromatographyonline.findanalytichem.com/lcgc/Combating-Terrorism-with-Mass-Spectrometry-mdash-S/ArticleStandard/Article/detail/607082?ref=25 In this article, we review the capabilities and challenges of deploying mass spectrometry (MS) to homeland security screening requirements. The fidelity of MS for chemical analysis is well accepted; however, the effectiveness of an overall system in real-world work environments depends on solving big challenges in sample collection, speed of analysis, and ease-of-use, and maintaining continuous reliability. We compare MS to the more commonly used security method of ion mobility spectrometry (IMS) and illustrate the significant improvements that MS can provide. We then describe one of the first introductions of MS in homeland security, that being the screening of people for concealed explosives using a walk-through portal device. 2009-07-01T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/272009/607082null XML http://chromatographyonline.findanalytichem.com/lcgc/HPLC-2009-Wednesday-Morning/ArticleStandard/Article/detail/605611?ref=25 Wednesday Morning in Dresden 2009-07-01T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605611null HTML http://chromatographyonline.findanalytichem.com/lcgc/Features/HPLC-2009-Show-Dailies/ArticleStandard/Article/detail/607343?ref=25 Stay up to date with HPLC 2009 by reading our show dailies. The whole lot now online! 2009-06-30T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/272009/607343/hplc1_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/272009/607343null HTML http://chromatographyonline.findanalytichem.com/lcgc/HPLC-2009-Tuesday-Afternoon/ArticleStandard/Article/detail/605610?ref=25 The Tuesday afternoon sessions of HPLC 2009 2009-06-30T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605610/hplcta_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605610null HTML http://chromatographyonline.findanalytichem.com/lcgc/HPLC-2009-Tuesday-Morning/ArticleStandard/Article/detail/605608?ref=25 HPLC 2009, Day Two 2009-06-30T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605608/hplctm_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605608null HTML http://chromatographyonline.findanalytichem.com/lcgc/HPLC-2009-Monday-Morning/ArticleStandard/Article/detail/605346?ref=25 What's happening at HPLC 2009? 2009-06-29T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605346/hplcmm_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605346null HTML http://chromatographyonline.findanalytichem.com/lcgc/HPLC-2009-Monday-Afternoon/ArticleStandard/Article/detail/605348?ref=25 What's going on in Dresden? 2009-06-29T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605348/hplcma_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605348null HTML http://chromatographyonline.findanalytichem.com/lcgc/Industry%3A+Pharmaceutical/Study-of-drug-quality-in-the-Indian-market/ArticleStandard/Article/detail/606025?ref=25 India is an increasingly influential player in the global pharmaceutical market. A pilot study, conducted in two major cities in India, Delhi and Chennai, explored the proliferation of substandard and counterfeit drugs in the market and discussed how the Indian state and federal governments could improve drug regulation and regulatory enforcement to combat this. 2009-06-26T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606025null HTML http://chromatographyonline.findanalytichem.com/lcgc/Industry%3A+Pharmaceutical/SFC-for-Pharmaceutical-Analysis-mdash-Opportunity-/ArticleStandard/Article/detail/605249?ref=25 Meeting Review 2009-06-26T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605249/SFC_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605249null REMOTE http://chromatographyonline.findanalytichem.com/lcgc/An-Acetonitrile-Free-Chromatographic-Methodology-f/ArticleStandard/Article/detail/606462?ref=25 The global economic downturn, particularly the collapse of the automotive and associated industries, has caused an overall reduction in the demand for acrylonitrile. Since acetonitrile is obtained as a co-product in the production of acrylonitrile, the pharmaceutical, food, environmental, and chemical industries are experiencing an unprecedented acetonitrile shortage. This shortage has resulted in a sharp price increase, in some cases as much as 6-8 times; and the price is projected to remain high even after the production returns to normal. 2009-06-26T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606462null PDF http://chromatographyonline.findanalytichem.com/lcgc/The-Column-mdash-its-evolving/ArticleStandard/Article/detail/606506?ref=25 LCGC Europe?s digital magazine, The Column is now delivered twice monthly with a new screen-optimized format designed to improve readability. Covering topical issues, application-based articles, market reports, tips and tricks, opinion pieces and interviews, The Column is a great information resource helping readers achieve better day-to-day working practices. 2009-06-26T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606506null HTML http://chromatographyonline.findanalytichem.com/lcgc/Characterization-of-Polyacrylnitrile/ArticleStandard/Article/detail/603376?ref=25 In this application note GPC is used to easily and reliably analyze the molecular mass distribution of industrially produced polyacrylnitrile (PAN). Since this GPC application uses DMF and a small amount of lithium bromide as mobile phase, it will work on a standard HPLC system without any modifications. 2009-06-26T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/603376null PDF http://chromatographyonline.findanalytichem.com/lcgc/Industry%3A+Biological/British-research-council-launches-consultation/ArticleStandard/Article/detail/606023?ref=25 The Biotechnology and Biological Sciences Research Council (BBSRC) is launching a consultation on Next Generation Sequencing Technologies. The council is seeking comments from individuals and organizations from the UK?s academic and industrial research community about the potential impacts that these technologies can have on research in biosciences. 2009-06-26T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606023null HTML http://chromatographyonline.findanalytichem.com/lcgc/Industry%3A+Industrial/Chemical-companys-Asian-acquisitions/ArticleStandard/Article/detail/606024?ref=25 Speciality chemicals group LANXESS AG has made two acquisitions in Asia. The company will acquire the chemical businesses and assets of Gwalior Chemical Industries for €82.4 million. LANXESS will also acquire the business and production assets of Chinese-based Jiangsu Polyols Chemical Co. 2009-06-26T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606024null HTML http://chromatographyonline.findanalytichem.com/lcgc/Industry%3A+Pharmaceutical/Lab-services-provider-opens-new-facility/ArticleStandard/Article/detail/606022?ref=25 A provider of contract laboratory services and manufacturer of scientific instruments in the US, has opened a new European laboratory and office in Warwickshire, UK. 2009-06-26T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606022null HTML http://chromatographyonline.findanalytichem.com/lcgc/Industry%3A+Biological/Confirming-Peak-Identification-in-Bioanalytical-St/ArticleStandard/Article/detail/605245?ref=25 Achieving Product Ion Confirmation 2009-06-25T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605245/Rainville_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605245/rainvillets_flash.jpg REMOTE http://chromatographyonline.findanalytichem.com/lcgc/Products/Lightweight-and-portable-leak-detector/ArticleStandard/Article/detail/606083?ref=25 The Adixen ASM 310 helium leak detector, from Alcatel Vacuum Products, is described as a combination of portability with performance. 2009-06-24T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606083/alcatel1_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606083null HTML http://chromatographyonline.findanalytichem.com/lcgc/Products/Extra-smooth-microfluidic-pumping-system/ArticleStandard/Article/detail/606084?ref=25 Dolomite has launched the Mitos Syringe Pump Extra Smooth (XS), a pumping solution, which is claimed to make complex and powerful microfluidic technologies possible. 2009-06-24T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606084/dolomite_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606084null HTML http://chromatographyonline.findanalytichem.com/lcgc/Industry%3A+Industrial/Laboratory-Equipment-Qualification-Back-to-Basics/ArticleStandard/Article/detail/605244?ref=25 Understanding the qualification requirements of all laboratory equipment is key to ensuring regulatory compliance. 2009-06-24T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605244/Vosloo_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605244/Vosloots1_flash.jpg REMOTE http://chromatographyonline.findanalytichem.com/lcgc/Lorem-ipsum-dolor-sit-amet-consectetur-adipiscing-/ArticleStandard/Article/detail/606285?ref=25 Lorem ipsum dolor sit amet, consectetur adipiscing elit 2009-06-24T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606285null HTML http://chromatographyonline.findanalytichem.com/lcgc/Products/Genetic-analysis-system/ArticleStandard/Article/detail/606086?ref=25 Fluidigm Europe has announced the release of the EP1 system for genetic analysis, which it claims provides the efficiencies of integrated fluidic circuit (IFC)-based genotyping in a desktop-sized configuration. 2009-06-24T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606086/fluidigm1_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606086null HTML http://chromatographyonline.findanalytichem.com/lcgc/Products/Scanning-electron-microscope/ArticleStandard/Article/detail/606085?ref=25 FEI has released the Quanta 50 Series scanning electron microscope (SEM), which is reported to offer a combination of performance and versatility over an extraordinary range of samples. 2009-06-24T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606085/fei_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/606085null HTML http://chromatographyonline.findanalytichem.com/lcgc/Technology-Forum-Pharmaceutical-Analysis/ArticleStandard/Article/detail/605745?ref=25 Joining us for this discussion are Jim Koers and Nick Duczak of Thermo Fisher Scientific; Phillip DeLand of Dionex Corporation; Roger N. Bardsley of Teledyne Tekmar; and Atis Chakrabarti of Tosoh Bioscience LLC. 2009-06-23T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605745/TechForumF_flash.jpg HTML http://chromatographyonline.findanalytichem.com/lcgc/Industry%3A+General/Market-Profile-UHPLC/ArticleStandard/Article/detail/605250?ref=25 Liquid Chromatography users are asked about their experience with UHPLC 2009-06-23T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605250/UHPLC_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605250null REMOTE http://chromatographyonline.findanalytichem.com/lcgc/Multimedia/Acetonitrile-Shortage-Smart-Solutions-to-Re-Optimi/ArticleStandard/Article/detail/605438?ref=25 The supply of HPLC-grade acetonitrile has been critically short for the past six months. Today the situation appears to be improving slightly, though pessimists predict that the shortage is here to stay. 2009-06-22T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605438/webcast_T_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605438null REMOTE http://chromatographyonline.findanalytichem.com/lcgc/Misc/Tips-amp-Tricks-GPCSEC/ArticleStandard/Article/detail/605251?ref=25 The effects of the flow-rate on GPC/SEC experiments are shown and we give you the tips to control them. 2009-06-22T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605251/TipsandTricks_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/262009/605251null REMOTE http://chromatographyonline.findanalytichem.com/lcgc/Letter-From-the-Editor-Taking-the-Temperature-of-t/ArticleStandard/Article/detail/605101?ref=25 As we move into the dog days of summer, the field of chromatography moves into one of its busiest times of the year, with a series of conferences and symposia on the way. 2009-06-19T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/252009/605101null HTML http://chromatographyonline.findanalytichem.com/lcgc/Approaches-to-Singleton-Achiral-Purification-of-Di/ArticleStandard/Article/detail/605099?ref=25 Several approaches for purifying difficult samples more efficiently for discovery research support are mentioned in this paper. These approaches use mass triggered HPLC on various specialty columns. 2009-06-19T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/252009/605099null REMOTE http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Vaccines-from-Polysaccharide-Materials/ArticleStandard/Article/detail/600014?ref=25 Size-reduced polysaccharides were analyzed as monovalent solutions by high-performance size exclusion chromatography (HPSEC) with multiangle light scattering (MALS) and refractive index (RI) detection to measure their absolute Mw. 2009-06-18T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/222009/600014null REMOTE http://chromatographyonline.findanalytichem.com/lcgc/Agilent-PodacstTip-6-Caring-for-your-UV-detector-F/ArticleStandard/Article/detail/604463?ref=25 Agilent Podacst:Tip #6: Caring for your UV detector Flow Cells 2009-06-18T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/252009/604463null HTML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/High-Throughput-Analysis-of-Metabolic-Stability-In/ArticleStandard/Article/detail/600015?ref=25 High-throughput screening (HTS) assays are commonly used in drug discovery to screen a large number of compounds. Compound optimization and sample analysis can limit throughput. This application note addresses LC?MS-MS optimization and sample analysis issues by using a multiplexed HPLC system and optimization software to speed throughput. 2009-06-18T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/222009/600015null REMOTE http://chromatographyonline.findanalytichem.com/lcgc/E-learning-demonstrations-at-HLPC-2009/ArticleStandard/Article/detail/603818?ref=25 LC•GC and Crawford Scientific are pleased to present a live preview of CHROMacademy at HPLC 2009 in Dresden. CHROMacademy is a primary on-line reference source for chromatographic and instrument troubleshooting. 2009-06-18T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/252009/603818/200x180_flash.jpg HTML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Routine-HPLC-Determination-of-Aminopyridine-Compou/ArticleStandard/Article/detail/600020?ref=25 4-aminopyridine (4-AP) is a potassium channel blocker granted orphan drug status for the treatment of multiple sclerosis. 4-AP and other aminopyridine isomers have been targeted for genotoxic effect studies. 2009-06-18T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/222009/600020null REMOTE http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Nucleotide-Bases-with-Excellent-Peak-Shapes/ArticleStandard/Article/detail/600017?ref=25 Using the Cogent Diamond Hydride column, these nucleotide bases are easily and reproducibly retained with 100% water as the mobile phase. The peak shapes are excellent compared to similar methods on end-capped columns. The method is easy to use and runs in under 5 min. 2009-06-18T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/222009/600017null REMOTE http://chromatographyonline.findanalytichem.com/lcgc/Europe/Danish-protein-centre-opens/ArticleStandard/Article/detail/603817?ref=25 Faculty of Health Sciences at the University of Copenhagen will open the doors of its new research centre, The Novo Nordisk Foundation Centre for Protein Research. The centre is the result of an historic donation from the Novo Nordisk Foundation, which in 2007 gave the university €80 million for its establishment. 2009-06-18T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/252009/603817null HTML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/2-Ways-to-Attain-Sharper-Peak-Shape-and-Higher-Sen/ArticleStandard/Article/detail/600016?ref=25 There are two simple things that analysts can do in order to meet the method detection limits (MDLs) requirements. 1) The solvent focusing technique allows for greater sensitivity without sacrificing resolution. 2) Proper GC column selection is critical in order to get the best peak shape with the lowest baseline. 2009-06-18T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/222009/600016null REMOTE http://chromatographyonline.findanalytichem.com/lcgc/Fast-Analysis-of-Water-Soluble-Vitamins-Thermo-App/ArticleStandard/Article/detail/603789?ref=25 Fast Analysis of Water-Soluble Vitamins-Thermo Application Note 2009-06-15T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/252009/603789null PDF http://chromatographyonline.findanalytichem.com/lcgc/NOVEL-MULTI-MODE-ODS-HPLC-COLUMN-Imtakt-Applicatio/ArticleStandard/Article/detail/603790?ref=25 NOVEL MULTI-MODE ODS HPLC COLUMN-Imtakt Application Note 2009-06-15T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/252009/603790null PDF http://chromatographyonline.findanalytichem.com/lcgc/ultrafleXtreme-Redefining-MALDI-Mass-Spectrometry-/ArticleStandard/Article/detail/603745?ref=25 ultrafleXtreme: Redefining MALDI Mass Spectrometry Performance-Bruker Application Note 2009-06-15T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/252009/603745null PDF http://chromatographyonline.findanalytichem.com/lcgc/Analysis-of-Albumin-Proteins-using-ProSEC-300S-Col/ArticleStandard/Article/detail/600868?ref=25 Albumins are found in animal tissue, blood plasma, in bacteria and in plants, and include many important proteins with key functions in the body. ProSEC 300S columns from Varian, Inc. are designed for protein analysis and may be used to investigate a wide range of globular proteins, including the albumins, by size exclusion chromatography (SEC). 2009-06-12T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/600868null PDF http://chromatographyonline.findanalytichem.com/lcgc/Europe/Award-nominees-sought/ArticleStandard/Article/detail/602569?ref=25 The Chromatography Forum of the Delaware Valley has begun seeking nominations for the 2011 Dal Nogare Award. The award is given each year to an individual who is an example of an outstanding scientist in the field of chromatography. 2009-06-12T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602569null HTML http://chromatographyonline.findanalytichem.com/lcgc/ThermodesporptionGCndashMC-analysis-of-ogranic-emi/ArticleStandard/Article/detail/601378?ref=25 In the automotive industry, thermodesorption is one of the approved testing methods identifying volatile and semi-volatile organic compounds in non-metallic car materials. Shimadzu combines thermodesorption system with GC–MS technology providing excellent precision and sensitivity? 2009-06-12T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601378null PDF http://chromatographyonline.findanalytichem.com/lcgc/Instant-column-connector-for-capillary-gas-chromat/ArticleStandard/Article/detail/602973?ref=25 Instant column connector for capillary gas chromatography-NLISIS Application Note 2009-06-11T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602973null PDF http://chromatographyonline.findanalytichem.com/lcgc/Small-scale-Purification-using-SFC-ELSD--Thar-Appl/ArticleStandard/Article/detail/602932?ref=25 Small-scale Purification using SFC ELSD -Thar Application Note 2009-06-11T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602932null PDF http://chromatographyonline.findanalytichem.com/lcgc/Research/Market-Profile-UHPLC/ArticleStandard/Article/detail/602607?ref=25 The market for high performance liquid chromatography continues to be one of the most dynamic markets of the analytical instruments industry. 2009-06-10T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602607/t0709_marketprofile_thumbnail_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602607/f0709_marketprofile_flash.jpg XML http://chromatographyonline.findanalytichem.com/lcgc/Determination-of-Phthalate-Varian-Application-Note/ArticleStandard/Article/detail/602645?ref=25 Determination of Phthalate-Varian Application Note 2009-06-10T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602645null PDF http://chromatographyonline.findanalytichem.com/lcgc/TSK-GEL-ODS-140HTP-23-m-Columns-for-the-Fast-and-R/ArticleStandard/Article/detail/602659?ref=25 Since the USA Patriot Act* has been enacted, many pharmaceutical companies have reformulated their over the counter (OTC) drug products with phenylephrine (a nasal decongestant) as a substitute for pseudoephedrine. 2009-06-10T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602659null PDF http://chromatographyonline.findanalytichem.com/lcgc/Technology-Forum-HPLC-Chiral/ArticleStandard/Article/detail/602665?ref=25 Joining us for this discussion are Nelu Grinberg of Boehringer Ingelheim Pharmaceuticals, Inc.;Todd Palcic of TharSFC, a Waters Company; and Jelena Kocergin of Regis Technologies, Inc. 2009-06-10T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602665/TechForumF_flash.jpg HTML http://chromatographyonline.findanalytichem.com/lcgc/Analysis-of-Bumetanide-Varian-Application-Note/ArticleStandard/Article/detail/602643?ref=25 Analysis of Bumetanide-Varian Application Note 2009-06-10T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602643null PDF http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Enabling-High-Pressure-Operation-via-a-Novel-Chann/ArticleStandard/Article/detail/601832?ref=25 Homeland security requirements create the need for portable mass spectrometers, with ion detectors capable of performing at elevated pressure. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601832null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/ProteinndashProtein-Interactions/ArticleStandard/Article/detail/601840?ref=25 Over-expression of recombinant proteins is commonly used for the production of protein reagents in industry and academia. Problems often occur relating to the stress put on the cells to deal with this huge increase in synthesis. Cellular proteins that are part of the protein synthesis machinery are often up-regulated under such conditions. Large quantities of the recombinant protein can be bound to these cellular proteins, making purification difficult. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601840null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Determination-of-UV-Absorbers-from-Sunscreens-by-U/ArticleStandard/Article/detail/601841?ref=25 Measure UV-absorbing compounds in consumer products and characterize the UV absorption spectra of the individual components by employing high-speed liquid chromatography with photodiode array detection. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601841null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Hyphenated-Techniques-as-Modern-Detection-Systems-/ArticleStandard/Article/detail/601820?ref=25 This article describes the use of combined ion chromatography-mass spectrometry (IC–MS) and ion chromatography-inductively coupled plasma mass spectrometry (IC-ICP–MS) to analyze potentially harmful compounds. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601820null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/ultrafleXtreme-Redefining-MALDI-Mass-Spectrometry-/ArticleStandard/Article/detail/601822?ref=25 The new ultrafleXtremeâ„¢ exceeds all current expectations of MALDI-TOF/TOF technology: A proprietary kHz smartbeam-IIâ„¢ MALDI laser integrated with a novel FlashDetectorâ„¢ and re-engineerd electronics makes it the only MALDI-TOF/TOF on the market to provide kHz acquisition in MS and MS-MS modes. It generates a new level of data quality in applications such as LC-MALDI proteomics, high resolution tissue imaging based biomarker discovery or Top-Down Sequencing. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601822null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Lowering-Detection-Limits-for-the-Analysis-of-PBDE/ArticleStandard/Article/detail/601829?ref=25 Polybrominated diphenyl ethers (PBDEs) are bioaccumulativetoxic compounds that are used as flame retardants. They have high boiling points and low thermal stability which makes analysis by GC–MS challenging. Additional bromines increase the thermal instability. A method was developed to analyze for polybrominated biphenyls using a large volume injection, short analytical column, and high mass tuning algorithm on the Thermo Scientific DSQ II with detection by EI Single Ion Monitoring (SIM). 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601829null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Fast-Analysis-of-Water-Soluble-Vitamins/ArticleStandard/Article/detail/601844?ref=25 This application note describes a fast LC method for the analysis of water-soluble vitamins using a Thermo Scientific Hypersil GOLD aQ column. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601844null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Rapid-Field-Detection-of-Chemical-Warfare-Agents-S/ArticleStandard/Article/detail/601849?ref=25 Rapid sample preparation using the CUSTODIONâ„¢ solid phase microextraction (SPME) syringe was applied to chemical warfare agents (CWAs), CWA simulants, by-products, and precursors. The samples were analyzed quickly and reliably with a sample-to-sample cycle time of less than 3 min using the GUARDIONâ„¢-7 portable capillary gas chromatograph toroidal ion trap mass spectrometer (GC–TMS). 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601849null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Direct-Determination-of-Mannose-6-Phosphate-Conten/ArticleStandard/Article/detail/601824?ref=25 D-Mannose-6-phosphate (M-6-P) is a terminal monosaccharide of some asparagine-linked (N-linked) oligosaccharides and is also part of an important intermediate in N-linked oligosaccharide biosynthesis. Some lysosomal glycoproteins require M-6-P terminated oligosaccharides for proper targeting and function. Lack of M-6-P or genetic defects in its synthesis or subsequent processing can result in a variety of diseases. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601824null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Flexible-Fused-Silica-Capillary-A-Discussion-of-St/ArticleStandard/Article/detail/601828?ref=25 Synthetic fused silica capillary tubing is a vital component in many scientific techniques. The general perception is that most laboratory glass products are fragile and easy to break. The opposite is true of fused silica capillary; with its protective coating it is both strong and durable when handled properly. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601828null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Total-Rapid-Prep-for-Same-Day-Turnaround-of-POPs-A/ArticleStandard/Article/detail/601837?ref=25 Human health not only depends on providing good medical care, but also on the priority given to prevent exposure to environmental and other health risks. Persistent Organic Pollutants (POPs) are organic compounds typically of anthropogenic origin that resist degradation and accumulate in the food chain and are associated with adverse effects on human health and the environment (1). Due to their toxicity to humans, at much lower concentration than other pollutants, it is important to monitor compounds like polychlorinated dioxins/furans PCDD/Fs, DLPCBs, BDEs, and PCNs. More sophisticated requirements are needed for their analysis. In the past, extraction and clean-up of POPs present in fish and biota samples were conducted with procedures such as Soxhlet extraction, acid digestion, and liquid-liquid extraction. The clean-up of these samples was accomplished through chromatographic columns using different types of adsorption media such as silica, alumina, and carbon. These analytical methods used for analysis.. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601837null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Glycerin-in-Biodiesel-by-Capillary-GC-Analysis/ArticleStandard/Article/detail/601839?ref=25 Biodiesel is much in the news today as an alternate fuel source that is safe and nontoxic. It is renewable, via farming and recycling, and is biodegradable. It is cleaner burning than petroleum-based gasolines, with virtually no sulfur and with no net carbon load to the atmosphere. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601839null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Two-Novel-Polysaccharide-Based-Chiral-Stationary-P/ArticleStandard/Article/detail/601821?ref=25 Polysaccharide-based chiral stationary phases (CSP) are successfully employed to achieve the majority of analytical and preparative enantioselective separations. However, there are a number of separations where significant modification or enhancement of selectivity is necessary. Two new columns, CHIRALPAK AY-H and CHIRALCEL OZ-H, have been introduced to meet these needs. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601821null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Virus-Particle-Characterization/ArticleStandard/Article/detail/601825?ref=25 Viruses are packets of infectious nucleic acid (either DNA or RNA) surrounded by a protective coat consisting of a large number of protein subunits. Since viruses can cause various diseases — some life-threatening — characterizing virus particles thoroughly in terms of their size distribution, aggregation, and absolute counts-per-unit volume is of extreme importance. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601825null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Separation-and-Scale-Up-of-an-Achiral-Compound-Uti/ArticleStandard/Article/detail/601835?ref=25 Although supercritical fluid chromatography (SFC) is generally regarded as a technique to separate enantiomers, it has also been proven in many laboratories as a fast approach for the purification of impurities in achiral samples. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601835null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Chlorinated-Solvents-and-Disinfection-By-product-A/ArticleStandard/Article/detail/601846?ref=25 Trace-level chlorinated hydrocarbon analyses using methods such as U.S. EPA Method 551.1 are important tools for assessing organochlorine contamination in water. The wide diversity of target organochlorine compounds can prove chromatographically challenging due mainly to their high volatility and limited retention. This application note shows the benefits of using an Agilent J&W HP-1ms Ultra Inert Capillary GC column as the primary column for detection in this dual-column analysis. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601846null XML http://chromatographyonline.findanalytichem.com/lcgc/Rapid-and-Accurate-Simultaneous-Quantitation-of-Lo/ArticleStandard/Article/detail/602529?ref=25 Rapid and Accurate Simultaneous Quantitation of Low Concentration Amphetamines and Metabolites from Urine using Strata?-X*-C and LC/MS/MS on Gemini C18 -Phenomenex Application Note 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602529null PDF http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/New-Innovations-for-EPA-8260-Analysis/ArticleStandard/Article/detail/601826?ref=25 Over the past 15 years, little has changed for the commercial environmental laboratory's ability to automate U.S. EPA Method 8260 for water and soil purge and trap analysis. As work loads have increased, reporting levels have decreased due to MS sensitivity improvements. However, it has become increasingly difficult for laboratories to run at high levels of productivity due to autosampler reliability, carryover, and internal standard reproducibility challenges. Each of these issues has been addressed in a new Centurion WS autosampler (see Figure 1) designed specifically for the commercial environmental laboratory. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601826null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Robust-and-Fast-Analysis-of-Four-Tobacco-Specific-/ArticleStandard/Article/detail/601833?ref=25 Tobacco Specific Nitrosamines (TSNA) are a group of carcinogens found only in tobacco products and are formed from nicotine and related alkaloids during the production and processing of tobacco and tobacco products (1). Due to their carcinogenic properties, efforts have been made to reduce TSNA levels in tobacco products. The project goal was to demonstrate a high throughput and sensitive method to monitor TSNA levels. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601833null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/A-Novel-Multi-Mode-ODS-Column-Which-Consists-of-An/ArticleStandard/Article/detail/601847?ref=25 Many polar compounds are difficult to retain by reversed-phase analysis without the use of ion-pair chromatography. We have overcome this disadvantage with a revolutionary phase structure called "Multi-mode ODS." This technology uses uniformly blended packing material consisting of two types of porous silica particles: 3 μm silica substituted with ODS+cation ligands and 3 μm silica substituted with ODS+anion ligands (Figure 1). This novel multi-mode ODS column, named Scherzo SM-C18, enables a multi-separation mode, consisting of: anion exchange, cation exchange, normal phase, and reversed-phase. In this article, we will validate this multi-separation mode by separating compounds which are difficult to separate on ODS column. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601847null XML http://chromatographyonline.findanalytichem.com/lcgc/Optimization-of-Wash-Elution-Conditions-Phenomenex/ArticleStandard/Article/detail/602528?ref=25 Optimization of Wash-Elution Conditions-Phenomenex Application Note 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602528null PDF http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/High-Performance-Ion-Exchange-Chromatography-for-A/ArticleStandard/Article/detail/601834?ref=25 Protein phosphorylation is one of the most prevalent intracellular protein modifications, regulating numerous cellular processes including cell differentiation, proliferation, and migration. Approximately 30% of cell proteins are phosphorylated at any given time and changes in protein phosphorylation often signal developmental or pathological disorders (1). To better understand the role of protein phosphorylation, it is important to separate the phosphorylated forms of a given protein. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601834null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Can-You-Use-Nitrogen-as-an-Alternate-Purge-Gas-for/ArticleStandard/Article/detail/601842?ref=25 Most U.S. Environmental Protection Agency (U.S. EPA) methods for analysis of volatile organic compounds (VOCs) specify purging with helium for 11 min at 40 mL/min, making purge-and-trap (P&T) one of the biggest consumers of helium in a laboratory. Compared to helium, nitrogen is abundant, inert, and can be purchased at affordable prices. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601842null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Simultaneous-Extraction-and-Quantitative-of-Morphi/ArticleStandard/Article/detail/601843?ref=25 Morphine is an effective pain-relieving drug that is primarily metabolized into morphine-3-glucuronide (M3G) and morphine-6-glucuronide (M6G). The highly potent M6G may have adverse effects, such as respiratory depression and renal failure, if accumulated in the body. As morphine abuse continues to affect modern society, an effective method must be established to analyse morphine and its structurally related compounds in biological fluid samples. In this work, a UPLC–MS-MS method was developed to separate six morphine-related compounds on a 2.1 × 100 mm, 1.8 μm ACQUITY UPLC HSS T3 column in a single run using an ACQUITY UPLC system connected to a fast-scanning triple-quadrupole MS detector (TQD). The method achieved adequate retention of these very polar compounds by reversed-phase (RP) chromatography in an 8-min total run time. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601843null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Determination-of-Iodide-and-Iodate-in-Seawater-by-/ArticleStandard/Article/detail/601845?ref=25 Iodine is an essential nutrient in seawater, seafood, and iodine-enriched foods, such as iodized table salt. The most common forms of iodine in the diet are iodide and iodate, with additional iodo-organic compounds providing a small fraction of the bio-available iodine. Iodine deficiency affects thyroid hormone production and leads to developmental diseases, goiter, and paralysis (1). 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601845null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Simultaneous-Separation-of-Pharmaceutical-Counteri/ArticleStandard/Article/detail/601851?ref=25 Salt formation is a critical aspect in drug development (1) and HPLC is an important tool for determination of pharmaceutical counterions. Reversed-phase columns often fail to retain very hydrophilic counterions. Ion chromatography (IC) is preferred for selective and sensitive screening of cationic and anionic pharmaceutical counterions (2), usually with multiple runs. Positive and negative counterions can be separated simultaneously using a zwitterionic HILIC column using an evaporative light scattering detector (3). However, the zwitterionic column method has limitations: 1) limited to HILIC mode; 2) ions are retained as salts rather than via ion-exchange, making method development difficult; and 3) the method is not suitable for simultaneous separation of acidic, basic, and neutral analytes. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601851null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/TSK-GEL-ODS-140HTP-23-microm-Columns-for-the-Fast-/ArticleStandard/Article/detail/601823?ref=25 Since the USA Patriot Act* has been enacted, many pharmaceutical companies have reformulated their over the counter (OTC) drug products with phenylephrine (a nasal decongestant) as a substitute for pseudoephedrine. Phenylephrine comes as a tablet, a liquid, or a dissolving strip to take orally — all as a treatment for cold symptoms (1). Besides phenylephrine, most pharmaceutical formulations for common cold and sinus medications often contain multiple active ingredients to treat different types of symptoms in addition to numerous excipients. From an analytical perspective, the challenge is to develop chromatographic conditions that allow quantitative analysis of a variety of excipients that vary widely in hydrophobic properties. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601823null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Automated-Handling-Techniques-for-the-Analysis-of-/ArticleStandard/Article/detail/601831?ref=25 In U.S. EPA Method 5035 there are two collection options for samples containing high levels of volatile organic compounds (VOCs). The first involves collecting a bulk soil sample in the field. In the lab, the bulk soil is dispersed in a water miscible solvent. Next, an aliquot of the solution is added to 5 mL of water and finally, the sample is purged. The second option is to collect a 5 g soil sample and add it to a pre-weighed vial containing a prescribed amount of water miscible solvent. An aliquot is then purged. Both of these processes are time consuming and have the potential to introduce human error both in the field and in the laboratory. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601831null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Profiling-the-Isomerization-of-Biologically-Releva/ArticleStandard/Article/detail/601836?ref=25 In the past few decades, single enantiomers and stereoisomers have overtaken achiral molecules in the percentage of approved drugs in the market. Because isomers can have different biological/pharmacological/toxicological properties, authorities, such as the European Pharmacopoeia and the FDA, have asserted escalated emphasis on controlling isomer content in drug compounds and require that stereoisomers, "be treated as separate drugs and developed accordingly" with rare exception. The separation and quantification of stereoisomers is therefore of great importance, especially when considering pharmaceutical compounds (1). 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601836null XML http://chromatographyonline.findanalytichem.com/lcgc/Products/Recycle-used-solvent/ArticleStandard/Article/detail/602351?ref=25 In response to the acetontrile shortage, Anachem has announced the availability of the SolventTrak Solvent Recycler, which the company claims provides a simple, practical, cost effective and environmentally friendly solution to help conserve acetonitrile supplies. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602351/anachem1_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602351null HTML http://chromatographyonline.findanalytichem.com/lcgc/Products/UHPLC-detector/ArticleStandard/Article/detail/602352?ref=25 ESA Biosciences has introduced the Corona ultra; a charged aerosol detector that the company claims is truly UHPLC compatible. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602352/ESA_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602352null HTML http://chromatographyonline.findanalytichem.com/lcgc/Products/QuEChERS-SPE-tubes-for-food-industry/ArticleStandard/Article/detail/602354?ref=25 Thames Restek has expanded its product portfolio with an increased range of award-winning QuEChERS SPE tubes. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602354/thames(2)_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602354null HTML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Reduced-Solvent-Usage-and-Increased-Throughput-for/ArticleStandard/Article/detail/601848?ref=25 Polynuclear aromatic hydrocarbons (PAHs) are carcinogenic condensed ring aromatic compounds widely found as trace pollutants in waters, wastes, air particulates, soil, and foods. PAHs can be monitored routinely using HPLC with a combination of UV and fluorescence detection as prescribed in EPA methods 550.1, 610, and 8310. Conventional HPLC analysis of 19 PAHs typically requires 20 min and uses 25 mL of acetonitrile. However, there is a continual drive to improve productivity and reduce solvent consumption and waste in chemical analysis. Using ultra high pressure LC (UHPLC) with sub-2 μm particle-size columns, we demonstrate a 3-fold improvement in throughput and a 90% reduction of mobile phase solvent in the determination of 19 PAHs. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601848null XML http://chromatographyonline.findanalytichem.com/lcgc/Non-Derivatized-Drugs-in-Urine-by-Automated-SPME-G/ArticleStandard/Article/detail/602524?ref=25 Non-Derivatized Drugs in Urine by Automated SPME GCxGC-TOFMS-LECO Application Note 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602524null PDF http://chromatographyonline.findanalytichem.com/lcgc/Products/Radio-HLPC-detector/ArticleStandard/Article/detail/602353?ref=25 The Model 5 version of the β-RAM radio HPLC detector from LabLogic Systems introduces a host of features that make it compatible with the latest methodologies, such as UPLC, Rapid Resolution LC and Fast LC. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602353/lablogic(2)_thumbnail.jpg http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602353null HTML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Identifying-Drugs-of-Abuse-Using-Gas-Chromatograph/ArticleStandard/Article/detail/601838?ref=25 Illegal drug use worldwide is at an all time high. Forensic laboratories are seeing increased sample loads creating an immediate need for fast and accurate analysis to positively identify confiscated materials in criminal investigations. This application highlights the value of gas chromatography with time-of-flight mass spectrometry (GC-TOFMS) for drug testing in forensic laboratories. A method was developed to successfully identify twenty drugs of abuse in 4.5 min. This GC-TOFMS method shows good chromatographic peak shape for even the most challenging drug analytes; even the peak shapes for amphetamine and methamphetamine were exceptional considering they were analyzed underivatized. The total ion chromatogram (TIC) for the twenty drug analytes is shown in Figure 1. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601838null XML http://chromatographyonline.findanalytichem.com/lcgc/Application+Notes/Comparison-of-Fully-and-Superficially-Porous-Parti/ArticleStandard/Article/detail/601850?ref=25 Since the introduction of high-performance liquid chromatography (HPLC) nearly 40 years ago, many improvements have been made to column stationary phases to achieve faster, more efficient separations. HPLC columns containing superficially porous (sometimes called fused-core) particles have recently gained increasing attention. Though this technology is not entirely new, it has been improved to the point where rapid, highly efficient separations can be achieved for some applications. 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601850null XML http://chromatographyonline.findanalytichem.com/lcgc/Continuous-Chromatographic-Separation-of-Salt-From/ArticleStandard/Article/detail/602474?ref=25 Continuous Chromatographic Separation of Salt From Sugars- Orochem Application Note 2009-06-09T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/242009/602474null PDF http://chromatographyonline.findanalytichem.com/lcgc/Europe/UHPLC-and-HPLC-resale-agreement/ArticleStandard/Article/detail/601462?ref=25 PerkinElmer and LEAP Technologies have entered into a resale agreement for ultra high performance liquid chromatography (UHPLC) and high performance liquid chromatography (HPLC) technology. 2009-06-05T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601462null HTML http://chromatographyonline.findanalytichem.com/lcgc/Optimizing-the-Analysis-of-Sugar-Alcohol-Excipient/ArticleStandard/Article/detail/601694?ref=25 Sugar alcohols require unique methods for analysis by HPLC due to their high polarity and lack of a UV-absorbing chromophore. USP methods for these separations take advantage of ion exclusion HPLC techniques. In this note, several different separations of sugar alcohols were performed us­ing ion exclusion Rezex RPM and RCU HPLC columns. 2009-06-05T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601694null PDF http://chromatographyonline.findanalytichem.com/lcgc/Europe/Collaboration-to-explore-FAIMS-with-TOF-MS/ArticleStandard/Article/detail/601464?ref=25 Owlstone Nanotech and Agilent Technologies have announced a phase one agreement to develop Owlstone's field-asymmetric ion mobility spectrometry (FAIMS) filter as a front-end separation module for Agilent's Accurate Mass time-of-flight mass spectrometers (TOF-MS). 2009-06-05T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601464null HTML http://chromatographyonline.findanalytichem.com/lcgc/Glyphosate-Retention-of-Extremely-Polar-Compounds-/ArticleStandard/Article/detail/601967?ref=25 Glyphosate: Retention of Extremely Polar Compounds-Microsolv Application NOte 2009-06-05T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601967null PDF http://chromatographyonline.findanalytichem.com/lcgc/Analysis-of-Albumin-Proteins-using-ProSEC-300S-Col/ArticleStandard/Article/detail/601691?ref=25 Varian, Inc. has published an application note entitled Analysis of Albumin Proteins using ProSEC 300S Columns. 2009-06-04T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601691null PDF http://chromatographyonline.findanalytichem.com/lcgc/An-Automated-High-Throughput-Sample-Fractionation-/ArticleStandard/Article/detail/601753?ref=25 The FMS Power-Prep? EPH Fractionation system performs fractionation and sample clean-up for up to 30 samples in less than 1 hour, producing the highest recoveries and best results for aliphatic and aromatic hydrocarbon content. 2009-06-04T04:00:00Z http://chromatographyonline.findanalytichem.com/lcgc/data/articlestandard/lcgc/232009/601753null PDF