High-Efficiency FAME Analysis Using Capillary GC - Essential fatty acids of primary interest were analysed with good resolution using a 60 m HP-88 column. - Chromatography Online
FindAnalytichem Custom Search
About Search
High-Efficiency FAME Analysis Using Capillary GC
Essential fatty acids of primary interest were analysed with good resolution using a 60 m HP-88 column.
Dec 2, 2007
By: Allen K. Vickers
The Application Notebook

Allen K. Vickers, Agilent Technologies, Folsom, California, USA.


Figure 1
Most authorities currently agree that saturated fatty acids are undesirable in one's diet and that hydrogenated fats can also be unhealthy. Whereas, some unsaturated fatty acids and particularly polyunsaturated fatty acids fall into the "healthy" category. Certain unsaturated fatty acids, notably the omega-3 and omega-6 fatty acids, have been said to have beneficial effects. It has therefore become increasingly important to develop analytical procedures that can unambiguously differentiate the various geometric and positional forms of dietary fatty acids. However, trans-unsaturated isomers, like saturated acids, need to be limited in our diet as they are linked to cardiovascular disease. Figure 1 and Tables 1 and 2 describe an analysis for the identification of fatty acids (as their methyl ester derivatives, FAMEs) in a commercially available fish oil/omega-3 and omega-6 dietary supplement using a 60 m HP-88 column. Good resolution is obtained for the essential fatty acids of interest, but also noted is the presence of several trans isomers, indicating that the source of the oils is perhaps adulterated or otherwise not of "virgin" quality.


Table 1


Table 2

Conclusion




A 60 m HP-88 column used for the identification of fatty acids in a commercially available fish oil/omega-3 and omega-6 dietary supplement obtained good resolution for the essential fatty acids of primary interest.

Agilent Technologies Inc.
2850 Centerville Road, Wilmington , Delaware 19808, USA
tel. +1 800 227 9770 fax +1 302 633 8901
E-mail: chem.@agilent.com

Website: http://www.agilent.com/chem/

ADVERTISEMENT

Rate this page
Your original vote has been tallied and is included in the ratings results.
View our top pages
 Average rating for this page is: 4.44
post a comment
Please complete the form below to post your comment.
Bold = Required
Email Address Your email address will NOT be published.
Display Name appears with your comment
City
State
Country
Remember me: YesNo
Comment read our privacy policy
Note: does not support HTML
All Comments submitted are subject to review, and may be delayed before posting. Comments will be posted at the discretion of the editor.
LCGC E-mail Newsletters
Select each newsletter you wish to subscribe for:
| Monthly
| Weekly
| Monthly
 Name:
 E-mail:
more info
Survey
What is your primary reason for attending analytica 2010?
Attending exhibition
Attending conference
Exhibiting at the show
A combination of all three
Other
Attending exhibition
0%
Attending conference
0%
Exhibiting at the show
0%
A combination of all three
0%
Other
0%
View Results
Source: The Application Notebook, 12/2/2007
Click here