Application Notes: Pharmaceutical - Chromatography Online
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Application Notes: Pharmaceutical

High Temperature Analysis of Nine Common Pharmaceuticals Using Sub-2 µm ZirChrom-PBD

September 1, 2009

In this application note we examine the effect of temperature on a sub-2 µm zirconia-based phase for the analysis of nine common pharmaceuticals.

Enhancement of UV Detection Sensitivity in SFC Using Reference Wavelength Compensation

September 1, 2009

Regulatory requirements for the identification, quantification, and control of impurities in drug substances and their formulated products are increasingly being explicitly defined, particularly through the International Conference of Harmonization (ICH).

Evaluation of a Multi-Mode ODS Column for the Separation of Polar Metabolites

September 1, 2009

The analysis of polar, ionic metabolites is important for drug discovery and biomarker research. Often times, it is necessary to separate these metabolites from each other and biological matrixes prior to MS detection (1).

Sensitive, High Resolution Mass Spec Analysis of Complex Pharmaceutical Mixtures after UHPLC Separation

September 1, 2009

In the pharmaceutical industry the increased efficiency brought about by new fast chromatography methods needs to be matched by commensurate mass spec capabilities. maXis UHR-TOF is the instrument of choice to keep up with fastest chromatography.

Increased Sensitivity, Improved Resolution and Faster Analysis Times For Compendial Methods: Determination of Impurities and Related Substances Using Newly Developed 2.6 µm Core-Shell Kinetex LC Columns

September 1, 2009

Recently a newly developed Kinetex 2.6 µm Core-Shell chromatographic particle has been commercialized that offers the performance benefits of sub-2 µm fully-porous particles but at substantially lower operating pressures.

Comparison of Liquid-Liquid Extraction (LLE) and Supported Liquid Extraction (SLE): Equivalent Limits of Quantitation with Smaller Sample Volumes

September 1, 2009

Traditional liquid-liquid extraction (LLE) can provide very clean extract. However, in many cases lower recoveries, labor-intensive liquid handling issues, and difficulty with automation can limit the success of LLE in sample preparation.

Trace-Level Aliphatic Amines in Cationic Drugs

September 1, 2009

The analytical challenge treated in the present work consists in determining sub-ppb concentrations of low-molecular-weight amines in the presence of strongly retained cationic drugs by using ion chromatography (IC) with upstream inline coupled-column matrix elimination (CCME).

EDTA by Anion Exchange HPLC

September 1, 2009

Hamilton Company is best known for manufacturing precision fluid measuring products, including syringes for HPLC and GC.

Simultaneous Separation of Pharmaceutical Counterions on a Reversed-Phase/Anion-Exchange/Cation-Exchange Tri-Mode HPLC Column

July 2, 2009

Salt formation is a critical aspect in drug development and HPLC is an important tool for determination of pharmaceutical counterions.

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