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Calibration Curves III: A Different View

May 1, 2009
By:John W. Dolan

This is the third installment in a series of LC Troubleshooting columns that focus on calibration curves used for LC methods. Here, John Dolan considers some alternate ways to look at the data to determine if they appear to be normal or are trying to tell us something is amiss.

Evaporation-Free Extraction and Application in High-Throughput Bioanalysis by LC–MS-MS

May 1, 2009
By:Aimin Tan, Saleh Hussain, François Vallée

Presented here are four types of evaporation-free extractions that are widely applicable.

Effects of Sample-Dissolving Solvents and Sample Ionic Strength in Routine CE Operation

May 1, 2009
By:Kevin Altria

The ionic strength of a sample and the solvent used to dissolve it play a pivotal role in routine capillary electrophoresis (CE).

Stir Bar Sorptive Extraction (SBSE) and Headspace Sorptive Extraction (HSSE): An Overview

May 1, 2009
By:Carlo Bicchi, Erica Liberto, Chiara Cordero, Barbara Sgorbini, Patrizia Rubiolo

The guest columnists review SBSE and HSSE and examine their advantages and limitations, along with providing approaches to overcoming the latter.

A Quality-by-Design Methodology for Rapid LC Method Development, Part III

April 1, 2009
By:Michael Swartz, Ira S. Krull, Joseph Turpin, Patrick H. Lukulay, Richard Verseput

The guest columnists continue their examination of how statistically rigorous QbD principles can be put into practice.

New Chromatography Columns and Accessories at Pittcon 2009: Part II

April 1, 2009
By:Ronald E. Majors

Ron Majors brings readers the second part of his yearly review of all that was new and innovative at the annual Pittsburgh Conference.

Calibration Curves, Part II: What are the Limits?

April 1, 2009
By:John W. Dolan

In part two of this series, John Dolan looks at the signal-to-noise ratio and its relationship to uncertainty in a measurement.

Approaches to Singleton Achiral Purification of Difficult Samples for Discovery Research Support in the Pharmaceutical Industry

April 1, 2009
By:Tony Q. Yan, Jim Bradow, Shang-Poa Chang, Rob Depianta, Laurence Philippe

Several approaches for purifying difficult samples more efficiently for discovery research support are mentioned in this paper. These approaches use mass triggered HPLC on various specialty columns.

Alternative Futures: Biofuels and Mass Spectrometry

April 1, 2009
By:Michael P. Balogh

The use of mass spectrometry to study biofuels is examined and the advantages and disadvantages are explored.

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