Agilent Podcast Series:Podcast:Consumption of Acetonitrile by 50 percent - - Chromatography Online
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Tips and Tricks of HPLC Analysis Podcast Series – Trusted techniques you can use today

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In this 12 part podcast series, expert chromatographers from Agilent Technologies offer helpful hints that they have collected in their years of experience to address some of the most popular concerns and difficulties in HPLC analysis. Learn the best tips and improve your chromatography results today! A new tip will be provided the last week of every month.

Tip #10 Ensuring Retention Time Reproducibility and Acceptable RSD Values in Peak Areas
In this podcast Adam will follow on tips from epidsode 7, and the benefits of moving your chromatography from standard 5um particles across to smaller sub two micron particles.
Adam Woodhouse
LC Product Specialist
Agilent Technologies
Tip #9 Ensuring Retention Time Reproducibility and Acceptable RSD Values in Peak Areas
This podcast will teach you about ensuring retention time reproducibility and acceptable RSD values in peak areas.
Pat Muldoon
Field Service Engineer
Agilent Technologies
Tip #8 Determine the Cause and Correction of your HPLC's High Back Pressure.
This seminar will outline the different options for checking the high back pressure issues, and how to correct with simple steps.
John Palmer
LC Columns Product specialist
Agilent Technologies
Tip #7 Use Small Particles for more efficient separation across wide flow range
Many laboratories are facing increasing sample loads and trying to cope with HPLC methods written using conventional HPLC columns; typically 4.6mm X 15cm or 25cm packed with 5-um particles. These labs are trying to decide the appropriate methodology to decrease analysis times without sacrificing chromatographic performance. In this podcast you will hear about the use of smaller particle sizes for more efficient separations without a lot of work.
Dawn Stickle
LC Applications Engineer
Agilent Technologies
Tip #6: Caring for Your UV Detector Flow Cells
People often think about flow cells only when they are trouble shooting problems of their HPLC system. Maintenance of your UV flow cell is simple and every user of the HPLC system can do it. Find out how to apply these tips in your lab with this podcast.
Tony Brand
Applications Scientist
Agilent Technologies
Tip #5: Seeing Baseline Noise and Drift, Ghost Peaks or Negative Peaks with HPLC
Baseline problems can include noise, wandering, drift, ghost peaks, and negative peaks. These symptoms can be of both mechanical and chemical origins. Today’s podcast is dedicated to highlighting some tips that you can watch for, or apply in your lab to reduce or remove these anomalies.
Tony Brand
Applications Scientist
Agilent Technologies
Tip #4: The Importance of Collecting the Appropriate Number of Data Points Across a Peak for Accurate Results
This podcast will review how to improve your chromatograms with respect to data acquisition.
Tony Brand
Applications Scientist
Agilent Technologies
Tip#3: Running overnight? Simple ways to remember to calculate solvent consumption
This Podcast will review some great tools to help with your solvent usage and some tips to ensure the best results possible.
Michael Woodman
LC Applications Engineer
Agilent Technologies
Tip #2: Reduce potential of flow blockage by some simple best practices
Particles leading to blockage can come from sources located both outside and inside the LC system. This Podcast will review the key tips to help avoid these issues.
Bob Giuffre,
Applications Specialist in liquid chromatography,
Agilent Technologies
Tip #1: Reducing Your Consumption of Acetonitrile by 50%
With the recent shortage of Acetonitrile, learn how to implement inexpensive solutions to reduce your use of this HPLC solvent and save money, today.
Maureen Joseph
LC Columns Product Manager
Agilent Technologies


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